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Services

Transient protein
expression services:
3-week TAT

HTP mAb expression
>1000 mAb/week capacity

OmniCHO™ Expression of mAb panel
in 24-deep-well plate (n=3)

Data sheets

Asymmetric, bispecific &
Ab fragments in OmniCHO™

Titer (mg/L)
1109
%monomer
99.7
Titer (mg/L)
736
%monomer
93.4
Titer (mg/L)
360
%monomer
100.0
Titer (mg/L)
236
%monomer
98.0
Titer (mg/L)
501
%monomer
95.3
Titer (mg/L)
167
%monomer
96.1
Titer (mg/L)
872
%monomer
100.0
Titer (mg/L)
3779
%monomer
96.21

Overview

aFucosylated SUPERCELL™ host
engineered by CRISPR for enhanced
productivity and immediate program start

aFucosylated variant of SUPERCELL™ (stable CHO-K1 host) and OmniCHO™ (transient CHO-K1 host) developed by biallelic knockout of FUT8 using CRISPR

Both OmniCHO™ and SUPERCELL aFuc cell lines available for immediate service work

Wild-type OmniCHO versus aFucosylated OmniCHO monoclonal antibody pool titers

Data sheets

aFucosylated mAb
example (N-glycan)

N-glycan comparison

Side-by-side table summarizing WT vs aFuc percentages across key N-glycan species. Designed for fast scanning of the biggest deltas and quick validation of the overall %aFuc / %Fuc outcome.

N-Glycan WT mAb aFuc mAb
G0-N 4.6% 3.3%
G0 4.9% 70.6%
G0F 76.8% 0.7%
Man5 6.8% 5.8%
G1(6) 1.3% 13.0%
G1(3) 0.3% 5.3%
G2 0.0% 1.2%
G2F 5.3% 0.0%
%aFuc 17.8% 99.3%
%Fuc 82.2% 0.7%
Scientist inspecting protein expression samples in a laboratory Scientist inspecting protein expression samples in a laboratory

Analytics

End-to-End expression and protein characterization with a focus on quality

Our packages include industry-leading analytics to ensure your candidates have the best chance of clinical success:

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  1. In Vivo Ready Material

    All productions >10mL scale done with a standard delivered endotoxin levels of <0.1EU/mg and each sample is individually tested.

  2. Molecule Specific Workflows

    Platformed workflows for ultrahigh purity for mAbs, bsAbs high in heterodimer, multi-column purification processes, high yield antigen and non-mAb construct design

  3. Advanced In House Analytics

    Mass spec, custom ELISAs, custom cell based assays, SPR, BLI, DLS, DSF, and more

  4. Jumpstart Process Development

    Jumpstart upstream and/or downstream process development using OmniCHO rapid turnaround material generation and process screening for liability handling

The premier system for high-quality transient expression

Using our OmniCHO™ platform, we generate high-quality material in our SF Bay Area production facility for in-vitro and in-vivo studies, helping you rapidly, reliably, and cost-effectively advance mAb and non-mAb biotherapeutic candidates.

Some of the commonly requested Developability Packages we offer (custom packages per your requirements are also available):

Scale Number of Variants Expected Yield TAT Analytics
96w DWP 192 0.5-2mg 2 wks SDS-PAGE, SE-HPLC, BLI
24w DWP 48 4-8mg 2 wks SDS-PAGE, SE-HPLC, intact/reduced mass, SPR, cell based potency
30mL SF 30 15-60mg 2 wks SDS-PAGE, SE-HPLC, intact/reduced mass, ELISA potency, cell based potency
1L SF 10 0.5-2.5g 3 wks SDS-PAGE, SE-HPLC, intact/reduced mass, HCP ELISA
5L SF 5 2.5-10g 3 wks SDS-PAGE, SE-HPLC, intact/reduced mass, 3-column DSP, HCP ELISA
Scale 96w DWP 24w DWP 30mL SF 1L SF 5L SF
Number of Variants 192 48 30 10 5
Expected Yield 0.5-2mg 4-8mg 15-60mg 0.5-2.5g 2.5-10g
TAT 2 wks 2 wks 2 wks 3 wks 3 wks
Analytics SDS-PAGE, SE-HPLC, BLI SDS-PAGE, SE-HPLC, intact/reduced mass, SPR, cell based potency SDS-PAGE, SE-HPLC, intact/reduced mass, ELISA potency, cell based potency SDS-PAGE, SE-HPLC, intact/reduced mass, HCP ELISA SDS-PAGE, SE-HPLC, intact/reduced mass, 3-column DSP, HCP ELISA
Frequently asked questions

Protein expression
FAQs

Quick answers about transient protein expression services.

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Discuss your molecule with our scientists

We don’t workaround — we innovate
and deliver

Explore whether OmniCHO™ is the right expression platform for your specific protein format.

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