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Product

GOLDILOCKS™
stable CHO-K1 Kit

Pool predicts clone. Every molecule. Every time.

  • 7-14 g/L clone titers
  • >95% stability at 60 gen
  • 100-200 kb cargo capacity
  • 4-chain co-integration

Core bottlenecks

Why cell line development still stalls — and what GOLDILOCKS removes from the process

Traditional workflows still rely on workarounds: random integration can be unstable, codon optimization is inconsistent, and multichain molecules often require extra rounds of signal peptide and vector screening.

Even improved transposase approaches leave failure modes in place, adding cost and stretching timelines.

  1. Random Integration

    What happens with traditional CLD

    • Cassette rearrangements; regulatory elements drift from ORFs
    • Concatemer formation drives repeat-induced silencing
    • Integration sites unpredictable – many silenced
    • Pool titer doesn’t predict clone titer

  2. Standard Transposase Platforms

  3. The Optimization Gauntlet

How GOLDILOCKS Works

  • Co-Transfection

    • Transposase mRNA + pGL™ vector
    • Optimized HTP transfection into SUPERCELL™
    • Single vector design for all molecules
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  • Multi-Site Integration

    • Inserts intact cassette at 15-20 loci
    • Single copy per site – no concatemers
    • Biased toward active chromatin
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  • Stable Expression

    • Pool titers >14 g/L
    • 50% increase from pool to clone
    • No selection pressure required
    • Stable >120 generations
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Key advantages

GOLDILOCKS™ is the only transposase platform engineered to eliminate every failure mode of random integration at the source – not to work around them.

  • Single-copy, multi-site integration

    15-20 independent integration sites, one copy per site. Eliminates the concatemer-induced silencing that drives expression decline in random-integration cell lines.

  • Intact cassette architecture

    The entire expression cassette integrates as a single unit – regulatory elements stay linked to their ORFs. No rearrangements, no orphaned promoters, no broken stoichiometry.

  • Active-chromatin targeting

    Integration is biased toward transcriptionally active loci. Your gene lands where the cell is already willing to express – not in heterochromatic silencing zones.

  • Pool predicts clone

    Clones average 50% above pool titer with tight distribution. Screen fewer clones.

  • Universal vector – every modality

    Universal pLG™ construct handles mAbs, bispecifics, ADCs, Fc-fusions, nanobody fusions, enzymes, cytokines. No per-molecule optimization. Up to 100-200 kb cargo.

  • Built-in genetic stability

    >95% expression retained over 120+ generations without selective pressure.

Proof points

GOLDILOCKS data — titers,
predictability, stability

Stable pool titers across 6 representative programs:Fc-fusion, nanobody fusion, 4-chain bispecific, lipoprotein, and two mAbs. All ≥7 g/L.

Pool titers reliably predict clone performance, reducing the number of clones that need to be screened.

Expression remains stable through extended culture without ongoing selection pressure.

How to Access GOLDILOCKS

GOLDILOCKS™ options — start with Pool Evaluation, then choose path

  • Pool evaluation

    Prove GOLDILOCKS first

    Test the platform before committing to a path:

    • 5-week stable pool + fed-batch COA
    • Accessible entry-point pricing
    • Pool preserved at Celltheon
    • Same path for Kit or Service

    Ideal for: platform evaluation, early biology readouts, molecule de-risking.

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  • License the kit

    Run GOLDILOCKS in your own lab

    For groups with in-house CLD infrastructure

    • Transposase mRNA + pGL™ vector
    • SUPERCELL™ CHO-K1 host
    • ELIXIR™ media + feed protocols
    • SOPs, training, and tech support

    Ideal for: labs running 5+ CLD programs/year, platform CDMOs, scaled biotech R&D.

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  • Full-service CLD

    We run GOLDILOCKS for you

    For groups with in-house CLD infrastructure

    • Transposase mRNA + pGL™ vector
    • SUPERCELL™ CHO-K1 host
    • ELIXIR™ media + feed protocols
    • SOPs, training, and tech support

    Ideal for: most biotech programs; gene to clone with no in-house CLD burden.

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Frequently asked questions

Quick answers
to common questions

Quick answers about SUPERCELL™.

View more answers

SUPERCELL™ is a next-generation CHO-K1 host cell line purpose-built for difficult-to-express proteins. Derived from the original Puck laboratory CHO-K1 lineage, SUPERCELL™ delivers superior expression of challenging molecule formats—including scFvs, bispecifics, and non-mAb therapeutics—with higher yields and improved product quality compared to standard CHO-K1 platforms.

Discuss your molecule with our scientists

We don’t workaround — we innovate
and deliver

Explore whether OmniCHO™ is the right expression platform for your specific protein format.

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