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Services

Protein analytics
services

Analytics that change your process – not just measure it.

  • Closed-loop
    in-house
  • 50+ assays
    in-house
  • Custom methods
    on request
  • Standalone
    or bundled

Closed loop

Why closed-loop analytics keeps decisions connected — instead of sending data into a broken workflow

Traditional analytics providers measure samples and return data, but they don’t control what happens next.

Celltheon keeps analytics connected to the upstream process, so insights can immediately inform expression, purification, characterization, and optimization decisions.

Send-Out CRO Model

  • Sample ships out, data ships back – round trip costs weeks
  • Methods aren’t standardized to your upstream process vendor
  • No path to act on the data without re-contracting upstream
  • Process changes happen elsewhere – analytics is just a checker

Celltheon Closed-Loop Model

  • Gene → expression → analytics → process modification, all in-house
  • Methods tied to the molecule we made on the platform that expressed it
  • See the data, change the upstream/downstream, re-measure in days – not months
  • Same team that designed your vector reads your EX trace
Celltheon laboratory scientists Celltheon laboratory scientists

The Six-Pillar Assay Menu

The six-pillar assay menu

Formulation & preformulation screening

Two coordinated phases: a structured DS cascade to identify the best buffer + excipient combinations, then stress and stability work to prove the lead candidate survives.

  • Phase 1

    3-Round DSF Cascade

    Iterative buffer → excipient → combinatorial screening

    Round 1 – Buffer + pH + Ionic Strength

    • Buffer systems across the relevant pH range
    • Buffer + ionic strength matrix; rank by Tm

    Round 2 – Excipient Screening

    • Sugars (sucrose, trehalose, sorbitol, mannitol)
    • Amino acids (arginine, proline, glycine, Glu + combos)
    • Cyclodextrins (HP-B, sulfobutylether-B, v)

    Round 3 – Combinatorial Optimization

    • Top excipients combined → top 3 leads to stability
  • Phase 2

    Stress Testing & Stability

    Find where the molecule breaks, prove the lead survives

    Forced Degradation Panel

    • Thermal stress at elevated temperature
    • Acid + base pH stress
    • Oxidative (H2O2, iron, AAPH)
    • Freeze-thaw cycling + mechanical agitation

    Stability Program on Lead

    • Real-time at recommended storage
    • Accelerated + stressed with humidity control

High-Concentration Formulation

  • Concentration capability

    • Transposase mRNA + pGL™ vector
    • Optimized Target concentrations >200mg/L (extendable higher)
    • Lead candidates split across top buffers from preformulation
    • Upconcentration via UF/DF and TFF
    • Defined-volume, defined-concentration DS outputHTP transfection into SUPERCELL™
    • Single vector design for all molecules
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  • Viscosity strategy

    • Viscosity measurement at clinical relevant concentrations
    • Viscosity-reduction excipients (arginine, proline, surfactants, sugar combos)
    • Iterative tuning to hit injection feasibility
    • Compatible with SC, IM, and high-dose IV routes
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  • High-concentration QC

    • SE-HPLC: aggregation propensity at concentration
    • DLS: sub-visible + colloidal stability
    • Viscometer: injection feasibility
    • SDS-PAGE: fragmentation under upconcentration stress
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Subtitle

Closed-loop
development in action

The same in-house team operates every step. Data from one analytical readout can trigger an upstream process change within the same week – not
a multi-vendor handoff.

Real iterations driven
by analytics:

  • N-glycan profile not optimal

    ELIXIR™ feed strategy modification

  • Aggregation at scale

    Downstream handling modification
    / formulation modification

  • Incomplete heterodimer formation

    Heterodimer isolation by multi-column chromatography

  • Truncation observed

    DSP conditions + harvest timing optimization

Available When You Need It

Process modifications:
tune the QA

Most analytical labs hand you a report. We hand you the report and a process change that moves the CQA toward your target.
Standard modifications we apply:

Modification What is Shift Downstream impact
Afucosylation Core fucose removal Enhanced ADCC potency
Sialylation Terminal sialic acid Improved PK half-life, reduced immunogenicity
Reduced mannosylation High-mannose species Lower non-specific clearance
Galactosylation control Modulate terminal Gal Complement activation tuning
Truncation inhibition Suppress C-/N-terminal clipping Improved primary structure homogeneity
Aggregation inhibition HMW species Higher monomer purity, better yield
Viscosity optimization of primary sequence Solution viscosity Enables high-concentration formulations

Engagement Models

Standalone analytics services with bundled pricing for multi-assay, CLD, and process development programs

  • Primary offer
    À la carte – primary offering

    Standalone analytics on your molecule

    Send us material, pick your methods, get the data

    • Pick any subset of the 50+ methods on the menu
    • Per-sample scaling – volume discounts apply
    • Quick-turn quote and sample receipt
    • No upstream commitment required

    Ideal for: standalone characterization, second-source confirmation, novel-modality method gaps

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  • Bundled with CLD/PD

    Discounted rate when analytics paired with program

    All in-program analytics flow through Celltheon’s labs

    • CQA profile tracked pool → clone → DS continuously
    • Process modifications applied based on emerging data
    • Discounted vs. à la carte (scoped in conversation)
    • Single team across CLD + analytics

    Ideal for: programs where Celltheon is also doing CLD or process development

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  • Custom method dev

    The assay you need isn’t on the menu yet

    Fit-for-purpose method development for bespoke assays

    • Common: novel binding kinetics, custom potency
    • Method development report + transferable SOP
    • Validated against your molecule + matrix
    • Becomes part of our menu after dev

    Ideal for: novel modality programs needing analytics that don’t exist platform-wide

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Frequently Asked Questions

Quick answers
to common questions

Quick answers about timelines, quality, and how to work with Celltheon.

View more answers

Discuss your molecule with our scientists

We don’t workaround — we innovate
and deliver

Explore whether OmniCHO™ is the right expression platform for your specific protein format.

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