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Product

CHO cell culture
media & feeds

Key benefits

Why chemically
defined matters

  1. Batch-to-Batch Consistency

    No animal components or hydrolysates — every lot delivers reproducible cell culture performance and product quality.

  2. Easier Downstream Purification

    Absence of undefined proteins simplifies purification and reduces contamination risk.

  3. Regulatory Compliance

    Eliminates animal-derived risks; supports compliance with GMP and Drug.

  4. Precise Process Control

    Known formulations allow scientists to pinpoint optimal nutrient concentrations and feeding strategies.

Key benefits

High-yield
protein production

  • >14 g/L

    mAb titers demonstrated in optimized fed-batch

  • 2x

    Protein titer increase vS. competing products

  • >90%

    Cell viability maintained throughout culture

  • 3-5x

    Fed-batch productivity boost with complementary feeds

Formulation basics

How leading media systems achieve high yields

  1. Nutrients

    Rich, balanced nutrient formulations delivering precise amino acid, vitamin, and trace element ratios optimized for recombinant protein expression.

  2. Cleanliness

    No growth factor inhibitors – absence of phenol red, and 2-mercaptoethanol reduces unwanted metabolic interference.

  3. Stability

    Optimized osmolality and pH buffering supports sustained culture for 14-21 day fed-batch processes.

  4. Compatibility

    Demonstrated consistent performance across CHO-GS, CHO-K1, CHO-DG44, and CHO-S cell lines.

One media for all workflows

Best-in-class platform provides an all-in-one solution for all stages of development workflows:

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Cloning medium

Supports GS-CHO cloning methods; sterility-tested.

Applications of ELIXIR™

ELIXIR™ applications across R&D to preclinical development — from early studies to production-ready material

  • Batch

    Simplified, single-inoculation process

    • Simple seeding and culture initiation
    • Ideal for early process screening
    • High-yield media deliver strong base titers
    • Minimal hands-on time required

  • Fed-batch

    Standard for biopharmaceutical manufacturing

    • Concentrated feeds replenish amino acids and vitamins
    • 3-5× productivity vs. batch conditions
    • Bolus feeding regimens (3% v/v) from day 3 every other day
    • Sustains peak cell density for 14-21 day cultures

  • Perfusion

    Continuous process for highest intensities

    • Verified against volumetric productivity (VP
    • Cell-specific perfusion rates (CSPR) tested
    • Steady-state culture with continuous media exchange
    • High or moderate cell density conditions both yielding high titers
    • Simulated perfusion in flask or continuous perfusion in bioreactor

Comparative pool titers

Scalability: from lab
to GMP manufacturing

Subtitle

Scalability by design

  1. Rational Media Design

    Sequential, complementary development methods ensure performance is maintained as volume increases.

  2. Liquid & Powder Formats

    Available in ready-to-use liquid and powder formats for flexibility at all scales.

  3. Process Parameter Robustness

    Media verified for pH, osmolality, endotoxin, bioburden, and component quantitative analysis.

  4. Platformed Processes for Rapid Scaleup

    Fully platformed and transferable process parameters for shake flask and bioreactor expression.

Protein Quality: Beyond Just Titer

  • High Purity Protein

    CHO media formulated for high monomer purity by SE-HPLC for all molecule formats and low concentration of charge variants by CEX-HPLC and icIEF

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  • Batch-to-Batch Reproducibility

    Chemically defined, growth-factor-free formulations eliminate serum variability, ensuring consistent critical quality attributes (CQAs) across production runs.

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  • Glycosylation Control

    Supplementation strategies (e.g., galactose-enriched feeds) can predictably increase N-linked galactosylation to meet specific product quality targets.

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Storage conditions

Practical parameters & storage guidance

  1. Powder Medium

    Store at 2-8°C, dry environment, protected from light. Reconstituted medium: 2-8°C, protected from light.

  2. Liquid Medium

    Store at 2-8°C. Once opened under sterile conditions, use within 1-4 weeks. Serum-free up to 2 weeks.

  3. Available Formats

    Liquid (500 mL, 1 L) and powder (5 L, 10 L, 25 L reconstituted volume) for process-scale flexibility.

  4. Rapid Prototyping

    Custom prototype formulations manufactured (up to 200 L) within 7 working days for process development needs.

Summary

The ideal CHO media system —synthesized from leading commercial platforms

  1. Chemically defined, ADCF, serum-free formulation ensuring batch-to-batch

  2. Demonstrated titers >14 g/L mAb in standard fed-batch

  3. Integrated system: one media for all steps

  4. Human-like glycosylation, preserved and tunable for biosimilars

  5. Scalable from 0.3mL deep well plates to 20,000 L+ bioreactors with consistent protein tier trends

  6. Flexible process modes: batch, fed-batch, and perfusion

  7. Full regulatory support: MP manufacturing, ISO certification

The right CHO media platform shortens time-to-market, maximizes product yield, and ensures quality from early development through commercial launch.

Frequently asked questions

Quick answers
to common questions

Quick answers about timelines, quality, and how to work with Celltheon.

Celltheon builds biotech technology platforms and delivers end-to-end solutions that bridge early drug development and manufacturing.

We support biotech and biopharma teams from discovery through preclinical development and manufacturing readiness.

Our offering spans DNA, protein production, engineering, developability, assays, stable cell lines, conjugation, and process development.

Both. Programs can use an integrated workflow or select the individual services and technologies they need.

Yes. Choose platform transfer to your team or a service model executed by Celltheon scientists.

We begin with your goals, molecule, timeline, and deliverables, then provide a clear technical plan and project scope.

We share relevant case studies, quality documentation, platform data, and a project-specific package aligned with your requirements.