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Services

Gene sequence on Monday.
Cloned DNA on Friday

Lead with the 1-week promise. Strongest for discovery groups where the DNA queue is the rate-limiter for the whole program.

  • 1-week
    turnaround
  • 1 µg → 1 mg
    per construct
  • Supercoiled
    mcDNA
  • Bridges to
    GMP-scale

Where Discovery Programs Stall on DNA

Why standard clonal DNA
delivery slows programs

Most discovery groups treat DNA as a commodity — order it, wait for it, hope it works.

Three places that model quietly costs you weeks and burns candidates that should have moved forward.

  1. Timelines Kill Iteration

    The queue problem

    • Bacterial clonal genes: 2–4 weeks per round
    • Every design iteration restarts the clock
    • Programs freeze design decisions early to avoid re-ordering
    • Discovery velocity is capped by DNA lead time

  2. Bacterial Baggage

    The quality problem

    • Plasmids carry antibiotic-resistance backbones
    • Endotoxin and host cell contaminants persist through prep
    • Silencing over time in stable and in vivo work

  3. Scale-Up Cliff

    The bridging problem

    • Research-grade DNA rarely matches GMP-grade consistency and purity
    • Scaleup beyond a few µg extends timelines even longer
Scientists working in the Celltheon laboratory

Three Advantages Baked Into Every Order

How PIXIE™
rebuilds the workflow

PIXIE™ is Celltheon’s proprietary cell-free enzymatic platform for supercoiled minicircle DNA. Applied at research scale, it collapses three of discovery’s slowest steps into one 1-week service.

  1. Pillar 1

    Cell-free → clean by construction

    • Zero bacterial fermentation — no HCP, no endotoxin, no host DNA
    • Choose whether you want antibiotic-resistance backbone in the final construct
    • Low CpG → reduced innate immune interference in assays
    • Predictable quality profile from first order to last
  2. Pillar 2

    Supercoiled mcDNA → better transfection

    • Native supercoiled topology preferred for transient expression
    • 2–5× higher expression vs relaxed / plasmid DNA
    • Compact expression cassette (backbone stripped)
    • Drop-in replacement for standard plasmid inputs
  3. Pillar 3

    1-week TAT → discovery keeps moving

    • Days 1–3: custom fragment synthesis from your sequence
    • Days 4–5: enzymatic assembly and plasmid prep
    • Sequence verification and QC included
    • Same molecule scales directly to PIXIE™ GMP

1-week delivery —
gene in, DNA out

Standard clonal DNA synthesis on the PIXIE™ service track. Fragment synthesis kicks off the day the sequence lands; the enzymatic PIXIE™ process delivers sequence-verified supercoiled DNA five business days later.

  1. Day 1

    Fragment Synth

    Sequence intake & synthesis start

    • Sequence check + codon review
    • Order fragments
    • Design QC applied
  2. Days 2–3

    Fragment Synth

    Fragment build & assembly prep

    • Custom gene fragments
    • Fragment QC
    • Ready for PIXIE™ assembly
  3. Day 4

    PIXIE™ Process

    Enzymatic assembly & amplification

    • Cell-free assembly
    • Cell-free amplification
    • PIXIE™ assembly cleanup
  4. Day 5

    PIXIE™ Process

    Purification, QC, release

    • Sequence verification
    • Purity check
    • Release + ship

Total turnaround:
5 business days · ~1 week gene-to-DNA
· Business-day milestones, no weekend charges

ALTHEA™
humanization funnel

Every parent mAb is expanded into a design pool of frameworks, then reduced by structural, sequence, and immunogenicity filters. The client sees only the ranked short list — not the compute in between.

  • Mini

    1–10 µg
    Turnaround
    1 week
    Common use
    Sequence verification, low-throughput screens, sanity checks

  • Midi

    10–100 µg
    Turnaround
    1 week
    Common use
    Small-format transfection panels, HTP screening plates

  • Maxi

    0.1–1 mg
    Turnaround
    1 week
    Common use
    Standard transient expression at 50 mL–500 mL scale

  • Mega

    1–10 mg
    Turnaround
    2 weeks
    Common use
    Large scale transient expression for animal studies

  • Giga

    10–100 mg
    Turnaround
    3 weeks
    Common use
    Large scale transient expression for NHP studies

Three Ways to Buy

Engagement models

Standalone clonal DNA is the default offer. Two adjacent models exist for programs that want their DNA to feed directly into downstream expression or scale-up.

  • Primary offer
    Standalone DNA — Primary

    Sequence to sequence-verified DNA

    The default 1-week service. Pick a yield tier, send the sequence.

    • 1-week turnaround end-to-end
    • 1 µg–100 mg per construct
    • Supercoiled mcDNA, sequence-verified
    • Volume pricing across multi-construct orders

    Ideal for: discovery teams whose bottleneck is DNA lead time

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  • DNA + Transient Expression

    One vendor, gene to expressed protein

    Clonal DNA feeds directly into a Celltheon OmniCHO™ transient run.

    • One contract, one contact, one QC chain
    • Material stays in-house end-to-end
    • Compressed gene-to-protein timeline
    • Ideal companion for developability screens

    Ideal for: programs running fast antibody / DEP campaigns

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  • Bridge to PIXIE™ GMP

    Same molecule, all the way to clinic

    Research-scale clonal DNA scales into full PIXIE™ GMP mcDNA production.

    • Same supercoiled topology at every scale
    • No re-cloning between research and GMP
    • Continuous documentation trail
    • De-risks IND-enabling DNA supply

    Ideal for: teams with strong in-house expression capacity

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Frequently Asked Questions

Quick answers
to common questions

Quick answers about SUPERCELL™.Quick answers about timelines, quality, and how to work with Celltheon.

View more answers

Discuss your molecule with our scientists

We don’t workaround — we innovate
and deliver

Explore whether OmniCHO™ is the right expression platform for your specific protein format.

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